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Jul 02, 2014Western blot analysis was performed. Approximately 100 mg of frozen cardiac tissue was dissected and immediately transferred to the RIPA buffer with a protease inhibitor cocktail, and homogenized on ice utilizing the homogenizer Silent Crusher. The homogenate was centrifuged (12000 RPM for 10 min at 4C).

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Experimental and human studies have demonstrated that innate immune mechanisms and consequent inflammatory reaction play a critical role in cardiac response to ischemic injury. Thus, the detection of immuno-inflammatory and cellular phenomena accompanying cardiac alterations during the early inflammatory phase of myocardial infarction (MI) may be an excellent diagnostic tool.

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500 g of ABW was first homogenised (Heidolph Brinkmann Homogenizer Silent Crusher) in 500 mL of distilled water, and incubated (24 h, 37C) to allow acting of tyrosinase. After .

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500 g of ABW was first homogenised (Heidolph Brinkmann Homogenizer Silent Crusher) in 500 mL of distilled water, and incubated (24 h, 37C) to allow acting of tyrosinase. After incubation the homogenate mixture was adjusted to pH = 10 by 1 M NaOH, and incubated

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Mar 15, 2012Skeletal muscle samples (2030 mg) were homogenized in 1 ml TRI Reagent using Heidolph Brinkmann's Silent Crusher M at 10,00015,000 rpm in Eppendorph RNase-free tubes. Separation was achieved by adding 0.2 ml of chloroform and precipitated using 0.5 ml of isopropanol.

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